FBI Handbook of Crime Scene Forensics (5 page)

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Authors: Federal Bureau of Investigation

Tags: #Business & Economics, #Infrastructure, #Social Science, #General, #True Crime, #Murder

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Lubricant Examinations

Lubricants encompass a range of substances, including petroleum products, natural fatty ester oils, and polyalkylene glycol oils. Automotive fluids (e.g., engine oil, brake fluid), certain cosmetics (e.g., bath oils, lotions), and some polishes contain lubricants. Lubricant examinations may also be conducted in sexual assault, vehicular homicide, or heavy-equipment sabotage cases.

Questions concerning lubricant evidence should be directed to 703-632-8441.
Follow the evidence submission directions, including
Requesting Evidence Examinations
and
Packaging and Shipping Evidence
.

  • Submit entire items (e.g., clothing) when possible. Air-dry the evidence, and package separately in paper bags.
  • Absorb suspected lubricants onto a clean cotton cloth or swab. Leave a portion of the cloth or swab unstained as a control. Air-dry the swab and pack in a heat-sealed or resealable plastic bag.
  • Submit suspected sources of lubricants for comparison examinations.
  • Package lubricants separately in leakproof containers.
Metallurgy Examinations
Comparison

Comparative examinations can determine whether two metals or metallic objects came from the same source or from each other. Metal comparisons can identify various surface and microstructural characteristics—including fractured areas, accidental damage, and fabrication marks—to determine whether the objects share a common origin. Moreover, the manufacturing methods used to produce an object can be determined. These manufacturing techniques can include casting, forging, hot and cold rolling, extrusion, drawing, swaging, milling, grinding, spinning, blanking, ironing, deep drawing, and others. Examinations can determine mechanical properties, such as the response of a metal to an applied force or load. Examinations also can determine chemical composition, including alloying and trace elements.

Broken or Mechanically Damaged Metal

The causes of failure or damage—such as the application of stress exceeding the tensile strength or yield limit of the metal; a material or manufacturing defect; or corrosion, cracking, or excessive service usage (fatigue)—can be determined. The magnitude of the force or load that caused the failure, how the force or load was transmitted to the metal, and the direction it was transmitted also can be determined.

Burned, Heated, or Melted Metal

Examinations can determine the approximate temperature to which a metal was exposed, the nature of the heat source, and whether a metal was in an electrical short-circuit situation.

Cut or Severed Metal

Examinations can determine the method by which a metal was severed, such as sawing, shearing, milling, turning, or thermal cutting. The nature of the thermal source (e.g., burner bar, electric arc welder) used can sometimes be determined.

Metal Fragments

Examinations can determine how metal fragments were formed. If fragments were formed by impulsive (short-duration, high strain rate) loading, an examination can determine whether an explosive was detonated and the magnitude of the detonation velocity. The nature of the object that was the source of the fragments often can be determined as well.

Specification Fraud and Noncompliant Materials

Metallurgical testing of materials can determine whether inferior components were substituted in contracting frauds. The composition and mechanical properties of materials can be examined to determine if the components meet contractual obligations or appropriate regulatory codes. Precious-metal content also can be determined.

Lamp Bulbs

Examinations can determine whether a lamp bulb was incandescent when its glass envelope was broken. Determinations also can be made as to whether a lamp bulb was incandescent when it was subjected to an impact force such as a vehicular collision. Such determinations can be made even if the glass was broken by the impact.

Watches, Clocks, and Timers

The conditions causing a watch, clock, timer, or other mechanism to stop or malfunction and whether the time displayed represents a.m. or p.m. (calendar-type timing mechanisms only) can be determined. The on/off condition of appliance timers damaged by a fire or explosion often can be determined.

Objects with Questioned Internal Components

X-ray radiography can nondestructively reveal the interior construction and the presence or absence of defects, cavities, or foreign materials. The position of on/off switches and other mechanical components can be determined.

Questions concerning metallurgy evidence should be directed to 703-632-8441.
Follow the evidence submission directions, including
Requesting Evidence Examinations
and
Packaging and Shipping Evidence
.

National Missing Person DNA Database Program Examinations

The National Missing Person DNA Database (NMPDD) Program is supported by both the DNA Analysis Unit I (DNAUI) and DNA Analysis Unit II (DNAUII). Nuclear DNA examinations are conducted in the DNAUI and mitochondrial DNA examinations are conducted in the DNAUII to support the NMPDD Program. Each unit has an NMPDD Program Manager who is available to answer any questions regarding case submission (contact information is listed below). Local, state, and federal law enforcement missing-person cases can be submitted directly to the FBI Laboratory or through the FBI field offices or resident agencies. All agencies must contact one of the FBI Laboratory’s NMPDD Program Managers before submitting samples. The submitting agency must have the necessary information and completed forms for sample submission. The FBI will perform mitochondrial DNA and nuclear DNA (STR) analyses on samples.

All samples submitted to the FBI Laboratory must have an incoming letter describing the samples submitted. A copy of the anthropology, odontology (dental), medical examiner and/or coroner, and law enforcement reports must be included with unidentified human remains samples submitted.

Contact either of the NMPDD Program Managers prior to submitting samples or for questions concerning samples.

  • Call
    703-632-7586
    for the DNAUI or
    703-632-7582
    for the DNAUII.
  • For FBI (internal) e-mail, write to Eric Pokorak for the DNAUI or John E. Stewart for the DNAUII.
  • For Internet e-mail, write to
    [email protected]
    for the DNAUI or
    [email protected]
    for the DNAUII.

Follow the evidence submission directions, including
Requesting Evidence Examinations
and
Packaging and Shipping Evidence
.

Samples from Biological Relatives of Missing Persons
  • Samples must be sent with a Consent and Information Form for the National Missing Person DNA Database (FD-935 form). A copy of the law enforcement report should accompany the samples submitted.
  • Collect samples in the following order of preference:
    1. Dried bloodstains.
    2. Buccal (oral) swabs.

Dried Bloodstains

Use the blood-cell collection kits that are available in FBI field offices or by contacting the NMPDD Program Managers at
703-632-7582
or
703-632-7586
.

Buccal (Oral) Swabs

  • Use sterile, cotton-tipped applicator swabs to collect four buccal (oral) samples. Rub the inside surfaces of the cheeks thoroughly (use two swabs on each side).
  • Air-dry the swabs and place them back into the original packaging or an envelope with sealed corners. Do not use plastic containers.
  • Identify each sample with the date, time, subject’s name, location, collector’s name, and case number.
  • Buccal samples do not need to be refrigerated.
Samples from Unidentified Human Remains

Call the Laboratory prior to submitting bones, teeth, or tissue.
The communication accompanying the evidence must reference the telephone conversation accepting the evidence.

Skeletal Samples

Anthropological examinations can determine whether skeletal remains are human or animal. Race, sex, approximate height, and stature at death can be determined from human remains.

  • Pick up samples with gloved hands or clean forceps.
  • Air-dry samples and place in paper bags.
  • Submit whole samples. Cutting skeletal samples increases the possibility of contamination.
  • If possible, submit three samples.
  • Submit skeletal samples with an anthropological report, preferably from an anthropologist certified by the American Board of Forensic Anthropology, or a medical examiner’s/ coroner’s report.
  • Submit skeletal samples in the following order of preference:
    1. Femur.
    2. Tibia.
    3. Humerus.
    4. Teeth, skull, and/or mandible.
    5. Hand and foot bones.
    6. Lower arm bone.
    7. Vertebrae.
    8. Ribs.

Teeth

Personal identifications can be made by comparing teeth with dental records and X-rays.

  • Pick up teeth with gloved hands or clean forceps.
  • Air-dry teeth and place in paper bags.
  • Submit teeth with an odontological report, preferably from an odontologist certified by the American Board of Forensic Odontology, or a medical examiner’s/coroner’s report.
  • Submit teeth in the following order of preference:
    1. Nonrestored molar.
    2. Nonrestored premolar.
    3. Nonrestored canine.
    4. Nonrestored front tooth.
    5. Restored molar.
    6. Restored premolar.
    7. Restored canine.
    8. Restored front tooth.

Tissue

Tissue samples usually will provide sufficient quantities of DNA for testing.

  • Pick up tissue with gloved hands or clean forceps.
  • Collect 1-2 cubic inches of red skeletal muscle.
  • Place tissue samples in a clean, airtight plastic container without formalin or formaldehyde and keep refrigerated or frozen.
  • Label the outer container “KEEP IN A COOL, DRY PLACE,” “REFRIGERATE ON ARRIVAL,” and “BIOHAZARD.”
  • Submit to the Laboratory as soon as possible.
Paint Examinations

The layer structure of a questioned paint sample can be compared with a known source from a suspect. The sequence, relative thickness, color, texture, number, and chemical composition of each of the layers can be compared.

The color, manufacturer, model, and model year of an automobile may be determined from a paint chip. Sourcing automotive paints is limited to factory-applied, original automotive paint.

Paint on safes, vaults, windowsills, and door frames can be transferred to and from tools. A comparison can be made between the paint from an object and the paint on a tool.

The Laboratory will not examine evidence to authenticate fine art or historical artifacts or to source spray paint or architectural paints.

Questions concerning paint evidence should be directed to 703-632-8441
. Follow the evidence submission directions, including
Requesting Evidence Examinations
and
Packaging and Shipping Evidence
.

  • Search the accident or crime scene and the personal effects of the victim(s) to locate paint fragments. Paint fragments often are found in the clothing of the hit-and-run victim(s). Submit the clothing. Paints can be transferred from one car to another, from car to object, or from object to car during an accident or a crime.
  • Control paint chips must be collected from the suspected source of the evidentiary paint. Controls must be taken from an area close to, but not in, any damaged area. If no damage is obvious, controls should be taken from several areas of the suspect substrate. Each layer can be a point of comparison. Controls must have all of the layers of paint to the substrate. This can be accomplished by the following:
    • Section an area of the painted surface.
    • Cut a paint sample from the surface using a clean, sharp instrument.
    • Lift or pry loosely attached chips or dislodge the paint by gently hitting the opposite side of the painted surface.
  • Package paint specimens in leakproof containers such as vials or pillboxes. Do not attach paint particles to adhesive tape. Do not use plastic bags, cotton, or envelopes to package paint specimens.
Pepper-Spray or Pepper-Foam Examinations

Oleoresin capsicum is a resin in various peppers. It may be used in self-defense sprays or foams. Ultraviolet dye (orange) and/or tear gas also may be in the sprays or foams. Items can be analyzed for the presence of oleoresin capsicum, dye, or tear gas.

Questions concerning pepper-spray evidence should be directed to 703-632-8441.
Follow the evidence submission directions, including
Requesting Evidence Examinations
and
Packaging and Shipping Evidence
.

  • Submit entire items (e.g., clothing) when possible. Air-dry the evidence, and package separately in paper bags.
  • Moisten a clean cotton cloth or swab with isopropanol (rubbing alcohol), and wipe over the suspected sprays or foams. Prepare a second moistened cloth or swab as a control. Air-dry the cloths or swabs and pack separately in heat-sealed or resealable plastic bags.
  • Submit spray canisters when possible.
  • Refer to
    Hazardous Materials Transportation
    when submitting pepper-spray canisters.

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